DNA restriction fragment length polymorphism analysis

In the human genome, an average of about 200 base pair mutation can occur (known as the neutral mutation), neutral mutations lead to inter-individual differences in the nucleotide sequence, referred to as DNA polymorphism. DNA polymorphism occurred in a number of restriction enzyme recognition site, the enzyme will have a length of DNA fragments of different fragments, known as restriction fragment length polymorphism (RFLP). RFLR by way of Mendelian genetics, in a particular family, if a particular gene and disease-specific fragment polymorphism closely linked, polymorphic fragments that can be used as a form of "genetic markers" to judge family members or Whether the fetus for disease gene carriers. Type A hemophilia, cystic fibrosis and phenylketonuria, and so on can be diagnosed using this method.