DNA ultracentrifugation

Modern separation and purification of plasmid DNA from E. coli isolated as representative of the overall situation in view of the bacteria (E.coli) in the molecular biology of the important status of E.coli isolated from purified DNA quality control has become in recent years Ultra-centrifuge technology is an important subject. The plasmid DNA of the rapid separation and purification of the ultra-centrifuge equipment (centrifuges speeding, turned and ancillary equipment) has put forward higher requirements.
E.coli is typical of the original cell biology, because of its lack of prokaryotic cell by cell with a unit from the kind of film composed of a variety of functions can be divided into specific components of the local and regional independent system of the endometrium, Therefore not included in his cell the cell (nucleus, endoplasmic reticulum, Golgi, the line and Latin America, lysosome, and so on). SEM micrographs showed that E.coli can have two distinct regions within each of the cytoplasm and nuclear in nature, in their thin layer around the outside of the cell membrane and very thick walls, some attached to the outside wall at the end of the free Flagellum. Plasmid DNA is located in the nuclear area, so as to the existence of thin filament, the filament-like in many cases is a very long loop of DNA fragments by a number of folded-up-close.
E.coli for the micro-structure of the question, in ultra-centrifugal separation and purification of plasmid DNA prior to the pre-order are:
E.coli → → cell wall with lysozyme to use surfactants such as SDS, Trit X-100 cell membrane, such as EE → pot with acetic acid to make DNA, RNA and protein most of the precipitation (90%).
Sediments can join the TE buffer (10m-MTris-HCL lmMEDTA, pH8.0) after the molecular sieve to live on protein to RNA; can also be used ultracentrifugation to-home, to RNA, or DNA-like class to DNA fragment .