Plasmid DNA ultracentrifugation separation method

Traditional methods of separation: A few years ago, because of equipment limitations, the plasmid DNA isolated by the general balance CsCl density centrifugation, since the formation of gradient. 10 ~ 12ml to one-tube capacity, for example, turned-off by the separation, 36.000rpm × 60 hours, with angle-separation turned 45, OOOrpm × 36 hours, including the former deceleration, spent a total of 130,000,000 to drive the Ministry of life , Who also used to drive the Department of 100,000,000 life, which is speeding at the time of the total life span of 100 centrifuges to 20,000,000,000 turn, no doubt the cost of each experiment is too high, and the amount of CsCl, your price and other factors, So that the separation and purification of such work has become very expensive experiment.