11.09.2008

Plasmid DNA of speeding centrifuge latest developments

(1) turn their back on the tube vertical speed centrifuge (Chin alloys or carbon fiber-made): From 1975 to the vertical pipe turned the world, in recent years, the major manufacturer of centrifuges developed in a vertical tube the other way round, single-tube capacity of 0.2 ml to 4Oml, the maximum speed from 50000rpm to 120000rpm, RCFmax up to 700, OOOXg, 90 during the development of new models and has been able to turn their back on plasmid DNA vertical centrifuge tube experiments done easier.
(3) near vertical turn their back on centrifuge tubes: In order to eliminate the vertical pipe used to turn their back on the plasmid DNA in the centrifugal wall formed by the Ministry of RNA precipitation of DNA has become the zone of pollution, but also to improve the general-turned beveled (Dip 25 • - 35 •) due to the settlement of a long distance, which separated from the shortcomings of a longer time in the last few years has developed a variety of vertical pipe near the other way round (that is, Near VerticalTube Rot, or turn their back on NVT referred to as Neo Angle Rotor, leave a small angle turn, referred to as NT). Centrifuge tube section of the central axis and the angle between the axis centrifuge drive in the 7.5 • - 10 •, from the speed 65000rpm to 120, OOOrpm, RCFmax can 646000 × g up to one-tube capacity from 2ml to 13.5ml. NVT (or NT) turned mainly to the development of the plasmid DNA and the separation of design, of course, it also applies to mitochondrial DNA, chromosome DNA, RNA and serum lipoprotein • separation and purification.
(3) non-continuous gradient separation step: school quality DNA isolation and purification method is the traditional gold formed tube from CsCl density gradient centrifugation, and so on balance, beginning of the centrifuge tube CsCl gold uniform density, samples of which uniformly distributed.